#1
Which method is commonly used to extract DNA from cells?
Electrophoresis
Polymerase Chain Reaction (PCR)
Gel Electrophoresis
Phenol-Chloroform Extraction
#2
What is the primary function of DNA extraction?
To amplify DNA
To determine the DNA sequence
To isolate DNA from other cellular components
To visualize DNA under a microscope
#3
Which of the following is NOT a step in the DNA extraction process?
Cell lysis
Centrifugation
DNA amplification
Precipitation
#4
What is the purpose of adding ethanol or isopropanol during DNA extraction?
To precipitate DNA
To amplify DNA
To lyse cells
To visualize DNA
#5
Which of the following is a common source of DNA for extraction?
Chloroplasts
Ribosomes
Mitochondria
Endoplasmic Reticulum
#6
What is the role of a buffer solution in DNA extraction?
To dissolve DNA
To maintain pH and prevent denaturation of DNA
To break down proteins
To precipitate DNA
#7
Which enzyme is commonly used to break down proteins during DNA extraction?
Ligase
Restriction enzyme
Protease
Polymerase
#8
What does PCR stand for in the context of DNA analysis?
Polymeric Chain Reaction
Polymerase Chain Reaction
Primary Chromosome Replication
Protein Coding Region
#9
Which technique is commonly used to analyze DNA sequences?
ELISA
Sanger sequencing
Western Blotting
PCR
#10
What is the purpose of running a DNA ladder alongside samples during gel electrophoresis?
To amplify DNA
To determine the DNA sequence
To estimate the size of DNA fragments
To visualize DNA under a microscope
#11
Which method is used to analyze DNA methylation patterns?
Bisulfite sequencing
Sanger sequencing
ELISA
PCR
#12
What is the function of a DNA polymerase enzyme in PCR?
To break down DNA strands
To amplify DNA
To separate DNA strands
To synthesize new DNA strands
#13
Which technique is used to visualize DNA fragments after gel electrophoresis?
Southern Blotting
Northern Blotting
Western Blotting
Eastern Blotting
#14
Which of the following techniques is used to analyze gene expression?
ELISA
PCR
Northern Blotting
Southern Blotting
#15
Which technique allows for the simultaneous detection of multiple DNA sequences in a single reaction?
PCR
Southern Blotting
Multiplex PCR
Sanger sequencing
#16
Which technique is used to analyze DNA-protein interactions based on their physical association?
Western Blotting
Southern Blotting
ChIP-seq
ELISA
#17
Which technique is used to study DNA-protein interactions based on their affinity?
Southern Blotting
Western Blotting
ChIP-seq
PCR