#1
Which method is commonly used to extract DNA from cells?
Phenol-Chloroform Extraction
ExplanationCommonly employed method involving phenol and chloroform to isolate DNA from cells.
#2
What is the primary function of DNA extraction?
To isolate DNA from other cellular components
ExplanationThe main purpose is to separate DNA from other cellular components for further analysis.
#3
Which of the following is NOT a step in the DNA extraction process?
DNA amplification
ExplanationDNA amplification is not a step in the DNA extraction process; it is a separate process.
#4
What is the purpose of adding ethanol or isopropanol during DNA extraction?
To precipitate DNA
ExplanationEthanol or isopropanol is added to precipitate DNA, separating it from the solution.
#5
Which of the following is a common source of DNA for extraction?
Mitochondria
ExplanationMitochondria are a common source of DNA for extraction due to their distinct DNA.
#6
What is the role of a buffer solution in DNA extraction?
To maintain pH and prevent denaturation of DNA
ExplanationBuffer solution helps maintain pH and prevents denaturation of DNA during extraction.
#7
Which enzyme is commonly used to break down proteins during DNA extraction?
Protease
ExplanationProtease is employed to degrade proteins and facilitate DNA extraction.
#8
What does PCR stand for in the context of DNA analysis?
Polymerase Chain Reaction
ExplanationPolymerase Chain Reaction is a technique to amplify DNA for analysis.
#9
Which technique is commonly used to analyze DNA sequences?
Sanger sequencing
ExplanationSanger sequencing is a common method for determining the sequence of DNA bases.
#10
What is the purpose of running a DNA ladder alongside samples during gel electrophoresis?
To estimate the size of DNA fragments
ExplanationDNA ladder helps estimate the size of DNA fragments in gel electrophoresis.
#11
Which method is used to analyze DNA methylation patterns?
Bisulfite sequencing
ExplanationBisulfite sequencing is a method used to study DNA methylation patterns.
#12
What is the function of a DNA polymerase enzyme in PCR?
To synthesize new DNA strands
ExplanationDNA polymerase in PCR synthesizes new DNA strands during the amplification process.
#13
Which technique is used to visualize DNA fragments after gel electrophoresis?
Southern Blotting
ExplanationSouthern Blotting is employed to visualize DNA fragments separated by gel electrophoresis.
#14
Which of the following techniques is used to analyze gene expression?
Northern Blotting
ExplanationNorthern Blotting is employed to analyze gene expression at the RNA level.
#15
Which technique allows for the simultaneous detection of multiple DNA sequences in a single reaction?
Multiplex PCR
ExplanationMultiplex PCR enables the simultaneous detection of multiple DNA sequences in a single reaction.
#16
Which technique is used to analyze DNA-protein interactions based on their physical association?
ChIP-seq
ExplanationChIP-seq analyzes DNA-protein interactions based on their affinity and physical association.
#17
Which technique is used to study DNA-protein interactions based on their affinity?
Southern Blotting
ExplanationSouthern Blotting studies DNA-protein interactions based on their affinity and physical association.