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DNA Extraction and Analysis Quiz

#1

Which method is commonly used to extract DNA from cells?

Phenol-Chloroform Extraction
Explanation

Commonly employed method involving phenol and chloroform to isolate DNA from cells.

#2

What is the primary function of DNA extraction?

To isolate DNA from other cellular components
Explanation

The main purpose is to separate DNA from other cellular components for further analysis.

#3

Which of the following is NOT a step in the DNA extraction process?

DNA amplification
Explanation

DNA amplification is not a step in the DNA extraction process; it is a separate process.

#4

What is the purpose of adding ethanol or isopropanol during DNA extraction?

To precipitate DNA
Explanation

Ethanol or isopropanol is added to precipitate DNA, separating it from the solution.

#5

Which of the following is a common source of DNA for extraction?

Mitochondria
Explanation

Mitochondria are a common source of DNA for extraction due to their distinct DNA.

#6

What is the role of a buffer solution in DNA extraction?

To maintain pH and prevent denaturation of DNA
Explanation

Buffer solution helps maintain pH and prevents denaturation of DNA during extraction.

#7

Which enzyme is commonly used to break down proteins during DNA extraction?

Protease
Explanation

Protease is employed to degrade proteins and facilitate DNA extraction.

#8

What does PCR stand for in the context of DNA analysis?

Polymerase Chain Reaction
Explanation

Polymerase Chain Reaction is a technique to amplify DNA for analysis.

#9

Which technique is commonly used to analyze DNA sequences?

Sanger sequencing
Explanation

Sanger sequencing is a common method for determining the sequence of DNA bases.

#10

What is the purpose of running a DNA ladder alongside samples during gel electrophoresis?

To estimate the size of DNA fragments
Explanation

DNA ladder helps estimate the size of DNA fragments in gel electrophoresis.

#11

Which method is used to analyze DNA methylation patterns?

Bisulfite sequencing
Explanation

Bisulfite sequencing is a method used to study DNA methylation patterns.

#12

What is the function of a DNA polymerase enzyme in PCR?

To synthesize new DNA strands
Explanation

DNA polymerase in PCR synthesizes new DNA strands during the amplification process.

#13

Which technique is used to visualize DNA fragments after gel electrophoresis?

Southern Blotting
Explanation

Southern Blotting is employed to visualize DNA fragments separated by gel electrophoresis.

#14

Which of the following techniques is used to analyze gene expression?

Northern Blotting
Explanation

Northern Blotting is employed to analyze gene expression at the RNA level.

#15

Which technique allows for the simultaneous detection of multiple DNA sequences in a single reaction?

Multiplex PCR
Explanation

Multiplex PCR enables the simultaneous detection of multiple DNA sequences in a single reaction.

#16

Which technique is used to analyze DNA-protein interactions based on their physical association?

ChIP-seq
Explanation

ChIP-seq analyzes DNA-protein interactions based on their affinity and physical association.

#17

Which technique is used to study DNA-protein interactions based on their affinity?

Southern Blotting
Explanation

Southern Blotting studies DNA-protein interactions based on their affinity and physical association.

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