Principles and Applications of Immunological Assays Quiz

Explore immunodiagnostics with questions on ELISA, flow cytometry, RIA, PCR, and more. Learn about techniques for detecting proteins, cells, and antibodies.

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Which of the following immunological assays measures the amount of antigen in a sample?

ELISA

Western Blot

Immunofluorescence

Flow cytometry

What is the basic principle behind ELISA (Enzyme-Linked Immunosorbent Assay)?

Detection of antigen-antibody interactions

Detection of radioactive isotopes

Measurement of light scattering

Measurement of electric charge

Which of the following is a key advantage of using flow cytometry in immunological assays?

High sensitivity

Simple operation

Low cost

Limited multiplexing capability

In which type of immunological assay are fluorescent dyes often used to label antibodies or antigens?

Western Blot

ELISA

Immunofluorescence

Radioimmunoassay

Which type of immunological assay involves separating proteins based on their size and charge?

Western Blot

ELISA

HPLC

SDS-PAGE

What is the primary detection method used in a radioimmunoassay (RIA)?

Enzyme activity

Fluorescence

Radioactivity

Light absorption

In which type of immunological assay is a capture antibody immobilized on a solid surface?

Western Blot

ELISA

Flow cytometry

Radioimmunoassay

Which of the following immunological assays is commonly used to detect and quantify specific proteins in a sample?

PCR (Polymerase Chain Reaction)

Western Blot

HPLC (High-Performance Liquid Chromatography)

ELISA

Which of the following is a disadvantage of using PCR (Polymerase Chain Reaction) in immunological assays?

Low sensitivity

Inability to detect DNA

Limited specificity

Risk of contamination

#10

Which of the following immunological assays is particularly useful for detecting small molecules such as hormones or drugs?

Western Blot

ELISA

Radioimmunoassay

Immunofluorescence

#11

Which immunological assay technique is used to separate and analyze proteins based on their mass and charge?

Western Blot

ELISA

Mass Spectrometry

Flow cytometry

#12

Which type of immunological assay is commonly used to identify and characterize specific cells in a heterogeneous cell population?

ELISA

Western Blot

Immunohistochemistry

Radioimmunoassay

#13

Which of the following is a limitation of using ELISA in immunological assays?

Low specificity

High cost

Inability to quantify antigens

Limited multiplexing capability

Principles and Applications of Immunological Assays Quiz

Which of the following immunological assays measures the amount of antigen in a sample?

What is the basic principle behind ELISA (Enzyme-Linked Immunosorbent Assay)?

Which of the following is a key advantage of using flow cytometry in immunological assays?

In which type of immunological assay are fluorescent dyes often used to label antibodies or antigens?

Which type of immunological assay involves separating proteins based on their size and charge?

What is the primary detection method used in a radioimmunoassay (RIA)?

In which type of immunological assay is a capture antibody immobilized on a solid surface?

Which of the following immunological assays is commonly used to detect and quantify specific proteins in a sample?

Which of the following is a disadvantage of using PCR (Polymerase Chain Reaction) in immunological assays?

Which of the following immunological assays is particularly useful for detecting small molecules such as hormones or drugs?

Which immunological assay technique is used to separate and analyze proteins based on their mass and charge?

Which type of immunological assay is commonly used to identify and characterize specific cells in a heterogeneous cell population?

Which of the following is a limitation of using ELISA in immunological assays?

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