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Principles and Applications of Immunological Assays Quiz

#1

Which of the following immunological assays measures the amount of antigen in a sample?

ELISA
Explanation

ELISA measures antigen amount.

#2

What is the basic principle behind ELISA (Enzyme-Linked Immunosorbent Assay)?

Detection of antigen-antibody interactions
Explanation

ELISA detects antigen-antibody interactions.

#3

Which of the following is a key advantage of using flow cytometry in immunological assays?

High sensitivity
Explanation

Flow cytometry offers high sensitivity.

#4

In which type of immunological assay are fluorescent dyes often used to label antibodies or antigens?

Immunofluorescence
Explanation

Immunofluorescence uses fluorescent dyes for labeling.

#5

Which type of immunological assay involves separating proteins based on their size and charge?

SDS-PAGE
Explanation

SDS-PAGE separates proteins by size and charge.

#6

What is the primary detection method used in a radioimmunoassay (RIA)?

Radioactivity
Explanation

RIA uses radioactivity for detection.

#7

In which type of immunological assay is a capture antibody immobilized on a solid surface?

ELISA
Explanation

ELISA immobilizes capture antibody on a solid surface.

#8

Which of the following immunological assays is commonly used to detect and quantify specific proteins in a sample?

Western Blot
Explanation

Western Blot detects and quantifies specific proteins.

#9

Which of the following is a disadvantage of using PCR (Polymerase Chain Reaction) in immunological assays?

Risk of contamination
Explanation

PCR has a risk of contamination.

#10

Which of the following immunological assays is particularly useful for detecting small molecules such as hormones or drugs?

Radioimmunoassay
Explanation

Radioimmunoassay detects small molecules like hormones.

#11

Which immunological assay technique is used to separate and analyze proteins based on their mass and charge?

Mass Spectrometry
Explanation

Mass Spectrometry separates proteins by mass and charge.

#12

Which type of immunological assay is commonly used to identify and characterize specific cells in a heterogeneous cell population?

Immunohistochemistry
Explanation

Immunohistochemistry identifies and characterizes specific cells.

#13

Which of the following is a limitation of using ELISA in immunological assays?

Limited multiplexing capability
Explanation

ELISA has limited multiplexing capability.

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