Laboratory Techniques in Biochemistry Quiz Explore essential lab techniques in biochemistry with this quiz. Test your knowledge on protein purification, chromatography, gel electrophoresis, and more!
#1
Western blotting
Gel electrophoresis
Mass spectrometry
Nuclear magnetic resonance spectroscopy
#2
Tris buffer
Ethanol
Sodium dodecyl sulfate (SDS)
Acetonitrile
#3
To quantify DNA concentration
To measure enzyme activity
To quantify protein concentration
To determine pH of a solution
#4
To quantify gene expression
To determine protein concentration
To measure enzyme activity
To identify specific antibodies or antigens
#5
Size exclusion chromatography
Affinity chromatography
Ion exchange chromatography
Gas chromatography
#6
Polymerase chain reaction (PCR)
Western blotting
Gel electrophoresis
High-performance liquid chromatography (HPLC)
#7
Gel filtration chromatography
Affinity chromatography
Ion exchange chromatography
High-performance liquid chromatography (HPLC)
#8
Bradford assay
Bicinchoninic acid (BCA) assay
Lowry assay
Gas chromatography
#9
X-ray crystallography
Western blotting
Enzyme-linked immunosorbent assay (ELISA)
Polymerase chain reaction (PCR)
#10
β-mercaptoethanol
Dithiothreitol (DTT)
Ethanol
Triton X-100
#11
To measure absorbance of samples
To disrupt cells and tissues
To visualize protein bands on a gel
To purify proteins
#12
Sodium chloride (NaCl)
Tris-HCl
Ethanol
Sodium dodecyl sulfate (SDS)
#13
To prevent denaturation of proteins
To inhibit protease activity and prevent protein degradation
To enhance protein binding to chromatography resin
To increase the solubility of proteins
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