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Viral Replication and Genetics Quiz

#1

Which enzyme is responsible for the synthesis of RNA from a DNA template during transcription?

RNA polymerase
Explanation

RNA polymerase catalyzes the formation of RNA strands using DNA as a template.

#2

Which of the following is NOT a step in the viral replication cycle?

Integration
Explanation

Integration is not a step in viral replication; it involves the insertion of viral DNA into the host genome, typical in retroviruses.

#3

Which of the following is NOT a type of viral genome?

Triple-stranded RNA
Explanation

Triple-stranded RNA is not a known type of viral genome; viruses typically have single or double-stranded RNA/DNA.

#4

What is the role of the viral capsid?

To protect the viral genome
Explanation

The viral capsid serves as a protective shell for the viral genome against degradation and environmental factors.

#5

Which of the following is a feature of the lytic cycle of viral replication?

Death of host cell upon release of viral particles
Explanation

In the lytic cycle, the infected cell is lysed, leading to the release of viral particles and often cell death.

#6

Which cellular organelle is involved in protein synthesis during viral replication?

Ribosome
Explanation

Ribosomes are responsible for protein synthesis, including viral proteins, during viral replication.

#7

Which of the following is a characteristic of RNA viruses?

They utilize reverse transcriptase for replication
Explanation

RNA viruses typically do not use reverse transcriptase; instead, they use RNA-dependent RNA polymerase for replication.

#8

What is the primary function of reverse transcriptase in retroviral replication?

It synthesizes DNA from RNA
Explanation

Reverse transcriptase converts viral RNA into DNA, facilitating integration into the host genome.

#9

Which of the following is NOT a mechanism by which viruses can enter host cells?

Phagocytosis
Explanation

Phagocytosis is a process by which cells engulf particles, not typically utilized by viruses for entry.

#10

Which enzyme is responsible for unwinding the double-stranded DNA during viral replication?

Helicase
Explanation

Helicase is an enzyme that unwinds the DNA double helix, enabling replication and transcription processes.

#11

What is a viroid?

A short, circular single-stranded RNA molecule
Explanation

Viroids are small infectious agents composed solely of short, circular RNA molecules, lacking a protein coat.

#12

What is the primary function of protease during viral replication?

To cleave viral proteins into functional units
Explanation

Protease cleaves long viral polyproteins into individual functional proteins, necessary for viral assembly and maturation.

#13

What is the primary difference between positive-sense and negative-sense RNA viruses?

Positive-sense RNA viruses have RNA genomes that can be directly translated into proteins
Explanation

Positive-sense RNA viruses have genomes that can be directly translated into proteins, while negative-sense RNA viruses require transcription into mRNA first.

#14

What is a provirus?

An inactive form of a virus that has integrated its genome into the host cell genome
Explanation

A provirus is a dormant viral genome integrated into the host cell genome, capable of reactivation.

#15

What is the significance of the 5' cap and poly-A tail in eukaryotic mRNA?

They protect mRNA from degradation
Explanation

5' cap and poly-A tail protect mRNA from enzymatic degradation, aiding in stability and translation.

#16

Which of the following is a characteristic feature of retroviruses?

They use reverse transcriptase to synthesize DNA from RNA
Explanation

Retroviruses use reverse transcriptase to convert their RNA genome into DNA, which can integrate into the host genome.

#17

What is the role of the envelope in some viruses?

To facilitate viral attachment to host cells
Explanation

The envelope, typically derived from host cell membranes, aids in viral attachment and entry into host cells.

#18

Which of the following is a mechanism used by viruses to evade host immune responses?

Mutation of viral surface proteins
Explanation

Mutation of viral surface proteins helps viruses evade immune recognition, facilitating survival and persistence.

#19

What is a viroplasm?

A specialized region in the host cell cytoplasm where viral components assemble
Explanation

A viroplasm is a specialized region in the host cell cytoplasm where viral components assemble and replication occurs.

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