Virology and Viral Cultivation Quiz

Agar plates are not typically used for viral cultivation, as viruses require living cells to replicate.

Viral cultivation aims at studying, diagnosing, and producing vaccines or antiviral drugs.

Viral cultivation is not aimed at eliminating viruses but rather studying them or producing vaccines.

Plaques represent areas where viral particles have lysed cells, forming visible clear zones.

Plaques are clear zones on a cell monolayer caused by viral lysis, indicating areas of viral infection.

Serum culture is not a method of viral culture; instead, cell culture and embryonated eggs are common techniques.

Plaque assay is a standard technique for quantifying and isolating viral particles.

Plaque assay helps in quantifying the number of infectious viral particles in a sample.

Cell culture involves growing cells in a controlled environment and is essential for many viral studies.

These agents ensure that only the intended viruses grow in the culture, preventing contamination.

Viral infection can cause cells to rupture and die, leading to cell lysis.

Gaseous media is not used for viral culture; instead, liquid and solid media are common.

Laboratory animals may be expensive, raise ethical concerns, and not all viruses infect them.

Embryonated eggs provide a suitable environment for growing viruses used in vaccine production.

Factors like host range, growth characteristics, and ethical considerations influence cell line selection for viral cultivation.

Real-time PCR allows for precise quantification of viral nucleic acids in a sample.

Cell culture can be expensive, labor-intensive, and may not support the growth of all viruses.

Viral isolation aims to separate and identify a specific virus from a sample, leading to the establishment of a pure culture.