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Specimen Collection and Handling Quiz

#1

Why is it important to follow proper labeling procedures for specimens?

All of the above
Explanation

Ensures accurate identification, tracking, and results interpretation.

#2

What is the primary reason for using a tourniquet during venipuncture?

To facilitate blood flow and aid in vein visualization
Explanation

Increases venous pressure and dilates veins.

#3

Which of the following is a suitable antiseptic for cleaning the site before venipuncture?

70% isopropyl alcohol
Explanation

Effective against skin flora without causing irritation.

#4

Which of the following is a suitable method for collecting a urine sample for culture?

Collect urine midstream
Explanation

Minimizes contamination from urethra or external genitalia.

#5

What is the preferred specimen for diagnosing malaria?

Capillary blood
Explanation

Higher parasite concentration in capillary blood.

#6

What is the recommended method for collecting a throat swab specimen for streptococcal culture?

Gently swabbing the posterior pharynx and tonsillar area
Explanation

Maximizes recovery of organisms without trauma.

#7

Which of the following tubes is used for collecting blood for a complete blood count (CBC)?

Lavender-top tube
Explanation

Contains EDTA to prevent clotting.

#8

Which of the following specimens should be collected first during a multiple specimen collection process?

Blood cultures
Explanation

Minimizes contamination from other samples.

#9

What is the appropriate method for collecting a wound swab specimen?

Gently swabbing the wound surface with a sterile swab
Explanation

Reduces contamination and trauma to wound.

#10

Which of the following specimens is typically collected in a blue-top tube?

Plasma for coagulation studies
Explanation

Contains sodium citrate to prevent clotting.

#11

What is the recommended method for collecting a sputum sample for acid-fast bacilli (AFB) testing?

Collecting the first morning sputum
Explanation

Contains higher concentration of pathogens.

#12

What is the recommended method for collecting a wound culture specimen?

Swabbing the wound surface with a cotton-tipped applicator
Explanation

Maximizes recovery of organisms.

#13

What is the appropriate method for collecting a stool sample for ova and parasite (O&P) examination?

Placing the sample in formalin immediately
Explanation

Preserves morphology of parasites.

#14

What is the recommended method for collecting a stool sample for Clostridium difficile testing?

Collecting the sample in a container with Cary-Blair transport medium
Explanation

Maintains viability of C. difficile.

#15

Which of the following is an appropriate step to take if a blood sample clots during collection?

Discard the sample and recollect
Explanation

Prevents erroneous results from clot interference.

#16

What is the appropriate technique for collecting a blood culture specimen?

Disinfecting the site with alcohol and allowing it to air dry before collection
Explanation

Minimizes risk of contamination.

#17

Which of the following anticoagulants is commonly used for collecting blood samples for coagulation studies?

Sodium citrate
Explanation

Prevents blood clotting by chelating calcium.

#18

Which of the following specimens requires chilling during transportation?

Serum for glucose testing
Explanation

Prevents glycolysis which can alter glucose levels.

#19

Which of the following is a suitable preservative for stool specimens in parasitology testing?

Formalin
Explanation

Preserves morphology of parasites.

#20

What is the appropriate action if a blood sample needs to be transported but cannot be delivered to the laboratory immediately?

Place the sample on ice packs
Explanation

Slows metabolic processes and minimizes cell degradation.

#21

Which of the following is a critical step in collecting a blood gas sample?

Removing air bubbles from the syringe
Explanation

Ensures accurate gas composition analysis.

#22

What is the recommended method for collecting a cerebrospinal fluid (CSF) sample?

Collecting CSF in a sterile container after the lumbar puncture needle is inserted
Explanation

Minimizes risk of contamination.

#23

Which of the following specimens requires protection from light during collection and handling?

Serum for vitamin B12 assay
Explanation

Prevents degradation of light-sensitive compounds.

#24

Which of the following additives is commonly found in gray-top tubes used for glucose testing?

Potassium oxalate
Explanation

Prevents glycolysis.

#25

What is the primary purpose of collecting a sputum sample for cytology?

To determine the presence of cancer cells
Explanation

Aids in diagnosis and staging of lung cancer.

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