#1
What is the purpose of cell lysis in protein purification?
To disrupt cell membranes and release proteins
ExplanationBreaks cell membranes to release proteins.
#2
What is the role of detergents in protein extraction and purification?
To remove lipids and other membrane components
ExplanationDetergents remove lipids & membranes.
#3
What is the purpose of ammonium sulfate precipitation in protein purification?
To precipitate proteins based on their solubility
ExplanationPrecipitates proteins based on solubility.
#4
What is the purpose of column chromatography in protein purification?
To separate proteins based on size
ExplanationSeparates proteins based on size.
#5
Which of the following is NOT a method of protein precipitation?
Chromatography
ExplanationChromatography separates, not precipitates, proteins.
#6
What is the purpose of size exclusion chromatography in protein purification?
To separate proteins based on size
ExplanationSeparates proteins by size exclusion.
#7
Which of the following is NOT a method used for protein quantification?
Ion exchange chromatography
ExplanationIon exchange chromatography purifies, doesn't quantify, proteins.
#8
What is the purpose of high-performance liquid chromatography (HPLC) in protein purification?
To separate proteins based on specific binding properties
ExplanationHPLC separates based on specific binding.
#9
Which of the following is a disadvantage of using ion exchange chromatography for protein purification?
It can lead to protein denaturation
ExplanationMay cause denaturation of proteins.
#10
What is the purpose of affinity chromatography in protein purification?
To separate proteins based on specific binding properties
ExplanationSeparates based on specific binding.
#11
Which of the following is a disadvantage of using protein A affinity chromatography for protein purification?
It is expensive
ExplanationProtein A affinity chromatography is costly.
#12
What is the purpose of dialysis in protein purification?
To remove small molecules and salts
ExplanationDialysis removes small molecules & salts.