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Microscopic Organisms and Laboratory Techniques Quiz

#1

Which of the following is a prokaryotic organism?

E. coli
Explanation

Prokaryotic organisms lack a nucleus and other membrane-bound organelles.

#2

What is the function of an autoclave in a microbiology laboratory?

To sterilize equipment and media
Explanation

Autoclaves use high-pressure steam to kill microorganisms and sterilize materials.

#3

What is the purpose of a streak plate in microbiology?

To culture bacteria
Explanation

Streak plates isolate bacterial colonies for further study and identification.

#4

Which of the following is an example of a Gram-negative bacterium?

Escherichia coli
Explanation

Escherichia coli is a well-known example of a Gram-negative bacterium.

#5

What is the function of a microscope's objective lens?

To magnify the image
Explanation

Objective lens increases the size of the specimen for better observation.

#6

Which staining technique is commonly used to visualize bacterial spores?

Endospore staining
Explanation

Endospore staining specifically targets bacterial spores for visualization.

#7

What is the purpose of the oil immersion technique in microscopy?

To increase resolution
Explanation

Oil immersion enhances resolution by reducing light refraction.

#8

Which staining technique is used to differentiate acid-fast bacteria from non-acid-fast bacteria?

Ziehl-Neelsen staining
Explanation

Ziehl-Neelsen staining specifically stains acid-fast bacteria for differentiation.

#9

Which of the following is a characteristic of archaea?

Commonly found in extreme environments
Explanation

Archaea thrive in extreme environments like high temperatures, acidity, or salinity.

#10

What is the purpose of a Petri dish in a microbiology laboratory?

To culture bacteria
Explanation

Petri dishes provide a surface for bacterial growth and observation.

#11

Which method is used for counting bacterial colonies on agar plates?

Streak plate method
Explanation

Streak plate method involves spreading bacterial sample to obtain isolated colonies for counting.

#12

Which microscope is commonly used for observing live cells in real-time?

Fluorescence microscope
Explanation

Fluorescence microscope excites fluorescently-labeled molecules for live cell observation.

#13

What is the purpose of a laminar flow hood in a microbiology laboratory?

To provide a sterile environment
Explanation

Laminar flow hoods maintain a sterile airflow to protect samples from contamination.

#14

Which method is used for the identification of bacteria based on their biochemical characteristics?

API test
Explanation

API test system identifies bacteria based on their biochemical reactions.

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