Microbiology Fundamentals and Laboratory Techniques Quiz

Prokaryotic cells lack a true nucleus.

Fungi have eukaryotic cellular organization.

Viruses cannot replicate outside of a host cell.

Bacteria lack a true nucleus.

Saccharomyces cerevisiae is a type of yeast, which is eukaryotic.

Protozoa are typically unicellular organisms.

Gram staining distinguishes between Gram-positive and Gram-negative bacteria based on cell wall structure.

Incubation refers to the process of encouraging growth rather than killing microorganisms.

Incubation is not a method of microbial control; it encourages growth.

TEM allows high-resolution imaging of internal cellular structures.

The streak plate technique is used to obtain isolated colonies for further study.

MacConkey agar inhibits the growth of Gram-positive bacteria, allowing for the isolation of Gram-negative species.

Gel electrophoresis sorts molecules based on size and charge through an electric field.

PCR involves denaturation, annealing, and extension, but not translation.

Negative staining highlights bacterial flagella by staining the background, leaving the flagella unstained.

Gram staining is used for cell wall differentiation, not cell counting.

FAME analysis characterizes microorganisms based on their fatty acid composition.

Metabolic assays like resazurin measure microbial growth by monitoring changes in metabolic activity.