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Discoveries in Molecular Biology Quiz

#1

Who discovered the structure of DNA?

James Watson and Francis Crick
Explanation

Watson and Crick proposed the double helix structure of DNA in 1953.

#2

What is the role of RNA polymerase in gene expression?

To transcribe RNA from DNA
Explanation

RNA polymerase catalyzes the synthesis of RNA from a DNA template during transcription.

#3

What is the role of DNA ligase in molecular biology?

To join Okazaki fragments
Explanation

DNA ligase plays a crucial role in sealing the gaps between Okazaki fragments during DNA replication.

#4

Which enzyme is responsible for adding nucleotides to the growing DNA strand during replication?

DNA polymerase
Explanation

DNA polymerase catalyzes the addition of nucleotides to the growing DNA strand during replication.

#5

What is the function of DNA helicase in DNA replication?

To unwind the DNA double helix
Explanation

DNA helicase is responsible for unwinding the DNA double helix during replication.

#6

What is the function of PCR in molecular biology?

To amplify DNA
Explanation

PCR (Polymerase Chain Reaction) is used to make multiple copies of a specific DNA segment.

#7

Which enzyme is responsible for unwinding DNA during replication?

Helicase
Explanation

Helicase is an enzyme that unwinds the DNA double helix during replication.

#8

Which technique is used to determine the sequence of nucleotides in a DNA molecule?

DNA sequencing
Explanation

DNA sequencing is a method to determine the order of nucleotides in a DNA molecule.

#9

What is the function of ligase in DNA replication?

To join Okazaki fragments
Explanation

Ligase seals the nicks between Okazaki fragments, creating a continuous DNA strand during replication.

#10

Which of the following is NOT a type of RNA?

Deoxyribonucleic RNA (dRNA)
Explanation

dRNA is not a recognized type of RNA; it is a term mixing DNA and RNA.

#11

Which technique is used to separate DNA fragments by size?

Gel electrophoresis
Explanation

Gel electrophoresis is a method to separate DNA fragments based on their size and charge.

#12

What is the function of reverse transcriptase in molecular biology?

To synthesize DNA from RNA
Explanation

Reverse transcriptase converts RNA into DNA, playing a key role in retroviral replication.

#13

What is the central dogma of molecular biology?

DNA transcription and translation
Explanation

The central dogma describes the flow of genetic information from DNA to RNA to protein.

#14

Which scientist discovered restriction enzymes?

Hamilton Smith
Explanation

Hamilton Smith discovered restriction enzymes in 1970.

#15

Which technique is used to analyze gene expression levels?

Northern blot
Explanation

Northern blot is a method to study the expression of specific genes by analyzing RNA.

#16

Who proposed the 'one gene, one enzyme' hypothesis?

George Beadle and Edward Tatum
Explanation

Beadle and Tatum proposed the idea that each gene controls the synthesis of one enzyme.

#17

What is the function of a plasmid in molecular biology?

To carry foreign DNA into a host cell
Explanation

Plasmids are small, circular DNA molecules that can carry foreign DNA into host cells.

#18

Who discovered the first restriction enzyme?

Werner Arber
Explanation

Werner Arber discovered the first restriction enzyme in 1968.

#19

What is the function of CRISPR-Cas9 in molecular biology?

To cut DNA at specific sequences
Explanation

CRISPR-Cas9 is a genome editing tool that can precisely cut DNA at specific locations.

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