#1
Who discovered the structure of DNA?
James Watson and Francis Crick
ExplanationWatson and Crick proposed the double helix structure of DNA in 1953.
#2
What is the role of RNA polymerase in gene expression?
To transcribe RNA from DNA
ExplanationRNA polymerase catalyzes the synthesis of RNA from a DNA template during transcription.
#3
What is the role of DNA ligase in molecular biology?
To join Okazaki fragments
ExplanationDNA ligase plays a crucial role in sealing the gaps between Okazaki fragments during DNA replication.
#4
Which enzyme is responsible for adding nucleotides to the growing DNA strand during replication?
DNA polymerase
ExplanationDNA polymerase catalyzes the addition of nucleotides to the growing DNA strand during replication.
#5
What is the function of DNA helicase in DNA replication?
To unwind the DNA double helix
ExplanationDNA helicase is responsible for unwinding the DNA double helix during replication.
#6
What is the function of PCR in molecular biology?
To amplify DNA
ExplanationPCR (Polymerase Chain Reaction) is used to make multiple copies of a specific DNA segment.
#7
Which enzyme is responsible for unwinding DNA during replication?
Helicase
ExplanationHelicase is an enzyme that unwinds the DNA double helix during replication.
#8
Which technique is used to determine the sequence of nucleotides in a DNA molecule?
DNA sequencing
ExplanationDNA sequencing is a method to determine the order of nucleotides in a DNA molecule.
#9
What is the function of ligase in DNA replication?
To join Okazaki fragments
ExplanationLigase seals the nicks between Okazaki fragments, creating a continuous DNA strand during replication.
#10
Which of the following is NOT a type of RNA?
Deoxyribonucleic RNA (dRNA)
ExplanationdRNA is not a recognized type of RNA; it is a term mixing DNA and RNA.
#11
Which technique is used to separate DNA fragments by size?
Gel electrophoresis
ExplanationGel electrophoresis is a method to separate DNA fragments based on their size and charge.
#12
What is the function of reverse transcriptase in molecular biology?
To synthesize DNA from RNA
ExplanationReverse transcriptase converts RNA into DNA, playing a key role in retroviral replication.
#13
What is the central dogma of molecular biology?
DNA transcription and translation
ExplanationThe central dogma describes the flow of genetic information from DNA to RNA to protein.
#14
Which scientist discovered restriction enzymes?
Hamilton Smith
ExplanationHamilton Smith discovered restriction enzymes in 1970.
#15
Which technique is used to analyze gene expression levels?
Northern blot
ExplanationNorthern blot is a method to study the expression of specific genes by analyzing RNA.
#16
Who proposed the 'one gene, one enzyme' hypothesis?
George Beadle and Edward Tatum
ExplanationBeadle and Tatum proposed the idea that each gene controls the synthesis of one enzyme.
#17
What is the function of a plasmid in molecular biology?
To carry foreign DNA into a host cell
ExplanationPlasmids are small, circular DNA molecules that can carry foreign DNA into host cells.
#18
Who discovered the first restriction enzyme?
Werner Arber
ExplanationWerner Arber discovered the first restriction enzyme in 1968.
#19
What is the function of CRISPR-Cas9 in molecular biology?
To cut DNA at specific sequences
ExplanationCRISPR-Cas9 is a genome editing tool that can precisely cut DNA at specific locations.