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Molecular Biology Concepts Quiz

#1

Which of the following is not a type of mutation?

Translation
Explanation

Translation is a step in protein synthesis, not a type of mutation.

#2

Which of the following is not a component of an amino acid?

Phosphate group
Explanation

Amino acids consist of an amino group, carboxyl group, hydrogen atom, and variable R group, not a phosphate group.

#3

What is the function of restriction enzymes in molecular biology?

To cut DNA at specific sequences
Explanation

Restriction enzymes cleave DNA at specific recognition sequences.

#4

Which of the following is not a nitrogenous base found in DNA?

Uracil
Explanation

Uracil is found in RNA but not in DNA.

#5

What is the central dogma of molecular biology?

All of the above
Explanation

Central dogma states that DNA is transcribed into RNA, which is translated into proteins.

#6

Which enzyme is responsible for unwinding DNA during replication?

Helicase
Explanation

Helicase unwinds the double helix structure of DNA.

#7

What is the role of tRNA in translation?

Carries amino acids to the ribosome
Explanation

tRNA brings amino acids to the ribosome for protein synthesis.

#8

What is the primary function of DNA polymerase during DNA replication?

Adds nucleotides to the growing DNA strand
Explanation

DNA polymerase adds nucleotides to the 3' end of the growing DNA strand.

#9

What is the role of RNA polymerase in gene expression?

Transcribes DNA into RNA
Explanation

RNA polymerase catalyzes the synthesis of RNA from a DNA template.

#10

What is the function of PCR (Polymerase Chain Reaction)?

To amplify DNA
Explanation

PCR is used to amplify a specific segment of DNA.

#11

What is the function of DNA ligase in DNA replication?

It joins Okazaki fragments together.
Explanation

DNA ligase seals the gaps between Okazaki fragments in the lagging strand.

#12

What is the role of the Shine-Dalgarno sequence in bacterial translation?

It helps in the binding of mRNA to the ribosome.
Explanation

Shine-Dalgarno sequence facilitates the binding of mRNA to the ribosome in bacteria.

#13

What is the significance of the genetic code being degenerate?

It allows for redundancy in the code.
Explanation

Degeneracy of the genetic code allows multiple codons to code for the same amino acid, providing redundancy and error tolerance.

#14

Which of the following is not a component of the lac operon in bacteria?

tRNA
Explanation

tRNA is involved in translation, not in the lac operon regulation.

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