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Microscope Basics and Handling Quiz

#1

Which part of the microscope is used for adjusting the focus?

Fine and coarse adjustment knobs
Explanation

Adjustment knobs control focus.

#2

What is the function of the condenser in a microscope?

To adjust the intensity of light
Explanation

Condenser controls light intensity.

#3

What is the function of the iris diaphragm in a microscope?

To control the intensity of light
Explanation

Iris diaphragm regulates light intensity.

#4

What is the function of the stage clips on a microscope?

To secure the slide in place
Explanation

Stage clips secure the microscope slide.

#5

What is the purpose of a coverslip in microscopy?

To support the slide
Explanation

Coverslip supports the microscope slide.

#6

What is the total magnification when using a 10x eyepiece and a 40x objective lens?

400x
Explanation

Multiply eyepiece and objective magnifications.

#7

Which type of microscope is commonly used for viewing live specimens?

Phase-contrast microscope
Explanation

Phase-contrast is ideal for live specimen observation.

#8

Which type of microscope is used for viewing objects in three dimensions?

Confocal microscope
Explanation

Confocal microscopy provides 3D views.

#9

What is the purpose of immersion oil in microscopy?

To improve resolution
Explanation

Immersion oil enhances resolution.

#10

What does the term 'working distance' refer to in microscopy?

The distance between the objective lens and the specimen
Explanation

Working distance is objective-to-specimen distance.

#11

What does the term 'numerical aperture' (NA) refer to in microscopy?

The ability of a lens to gather light
Explanation

NA measures light-gathering capability.

#12

Which microscope technique is best for observing the surface of a specimen with high resolution?

Scanning electron microscopy
Explanation

SEM provides high-res surface images.

#13

Which microscope technique utilizes fluorescence to visualize specific structures within a specimen?

Fluorescence microscopy
Explanation

Fluorescence microscopy uses fluorescence for visualization.

#14

Which microscope technique is suitable for observing internal structures of thick specimens without slicing them?

Confocal microscopy
Explanation

Confocal microscopy for thick specimen internals.

#15

Which microscope technique is used to observe the surface of non-conductive specimens?

Atomic force microscopy
Explanation

Atomic force microscopy for non-conductive surfaces.

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