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Microscope Anatomy and Usage Quiz

#1

What part of the microscope is used to adjust the amount of light passing through the specimen?

Diaphragm
Explanation

Adjusts light intensity passing through the specimen.

#2

Which part of the microscope holds the objective lenses and can be rotated to change magnification?

Nosepiece
Explanation

Holds objective lenses and facilitates magnification change.

#3

What is the function of the iris diaphragm on a microscope?

To adjust the intensity and size of the light beam
Explanation

Controls light intensity and size for optimal illumination.

#4

Which microscope technique is commonly used for observing living cells in culture?

Phase-contrast microscopy
Explanation

Commonly employed for observing living cells in culture.

#5

Which part of the microscope is used to support the slide containing the specimen?

Stage
Explanation

Supports the slide containing the specimen.

#6

Which type of microscope is commonly used to observe live specimens?

Fluorescence microscope
Explanation

Utilized for observing live specimens by detecting fluorescence.

#7

What is the function of the coarse adjustment knob on a microscope?

To focus the specimen under low magnification
Explanation

Used for initial focusing of specimen at low magnification.

#8

What is the purpose of immersion oil in microscopy?

To improve resolution
Explanation

Enhances resolution by reducing light refraction.

#9

Which microscope technique is commonly used to study the surface of a specimen in three dimensions?

Scanning electron microscopy
Explanation

Used for 3D surface analysis of specimens.

#10

Which microscope technique relies on the bending of light to create contrast in transparent specimens?

Phase-contrast microscopy
Explanation

Relies on light bending for contrast in transparent specimens.

#11

What is the purpose of the mechanical stage on a microscope?

To hold and move the specimen
Explanation

Facilitates specimen manipulation and positioning.

#12

What is the purpose of the immersion objective lens on a microscope?

To enhance resolution
Explanation

Enhances resolution by reducing light refraction.

#13

Which microscope technique is used to observe internal structures of a specimen without staining?

Phase-contrast microscopy
Explanation

Enables visualization of internal structures without staining.

#14

What is the maximum magnification achievable with a light microscope?

1000x
Explanation

Maximum magnification attainable with a light microscope.

#15

What does the term 'resolution' refer to in microscopy?

The ability to distinguish between two adjacent objects
Explanation

Capability to differentiate between closely spaced objects.

#16

Which microscope technique utilizes a beam of electrons to visualize specimens?

Transmission electron microscopy
Explanation

Utilizes electron beam for specimen visualization.

#17

Which type of microscope is suitable for observing the detailed structure of cell organelles?

Transmission electron microscopy
Explanation

Ideal for detailed observation of cell organelles.

#18

What is the primary difference between a compound microscope and a stereo microscope?

Depth perception
Explanation

Stereo microscope offers improved depth perception.

#19

Which microscope technique is best suited for observing fluorescently labeled molecules within a cell?

Fluorescence microscopy
Explanation

Optimal for observing fluorescently labeled molecules.

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