#1
Which of the following is NOT a primary nutritional requirement for microbial growth?
Oxygen
ExplanationMicrobes can be anaerobic and do not require oxygen for growth.
#2
Which of the following is a characteristic of a halophile?
Thrives in high salt environments
ExplanationHalophiles are organisms that thrive in high salt concentrations.
#3
Which of the following is NOT a method of sterilization?
Incubation
ExplanationIncubation is the process of maintaining a controlled environment for microbial growth, not sterilization.
#4
Which of the following is an example of a facultative anaerobe?
Salmonella enterica
ExplanationFacultative anaerobes can grow with or without oxygen.
#5
Which of the following is a method of measuring microbial growth?
Serial dilution and viable plate count
ExplanationSerial dilution and viable plate count involve diluting a sample and counting the number of colonies formed on agar plates to estimate the number of viable cells.
#6
What is the optimal pH range for most bacteria to grow?
pH 6-8
ExplanationMost bacteria grow optimally in a slightly acidic to neutral pH range.
#7
Which of the following is an example of an obligate anaerobe?
Clostridium botulinum
ExplanationObligate anaerobes cannot survive in the presence of oxygen.
#8
What is the term for a microorganism that grows best at low temperatures, typically between 0°C and 20°C?
Psychrophile
ExplanationPsychrophiles thrive in cold environments.
#9
What is the term for the process by which bacteria reproduce by dividing into two identical daughter cells?
Binary fission
ExplanationBinary fission is the primary method of bacterial reproduction.
#10
Which of the following is a source of micronutrients for microbial growth?
Vitamins
ExplanationVitamins are essential micronutrients required by microorganisms for growth and metabolism.
#11
Which of the following is NOT a method of measuring microbial growth?
Gram staining
ExplanationGram staining is a technique for classifying bacteria based on their cell wall structure, not for measuring growth.
#12
Which of the following is an example of a chemoheterotroph?
Fungi
ExplanationChemoheterotrophs obtain energy and carbon from organic molecules.
#13
Which of the following is a characteristic of a fastidious microorganism?
Requires complex nutritional requirements
ExplanationFastidious organisms have complex nutritional requirements and are difficult to culture.
#14
Which of the following is an example of an autotrophic microorganism?
Cyanobacteria
ExplanationAutotrophic organisms can produce their own food from inorganic sources like carbon dioxide.
#15
Which of the following is an example of a photoautotroph?
Chlamydomonas reinhardtii
ExplanationPhotoautotrophs use light energy to convert carbon dioxide into organic compounds for growth and energy.