Microbial Culturing and Analysis Quiz

Autoclaving is a method of sterilization using high pressure and temperature.

A pure culture contains only one species of microorganism, not multiple species.

Storing at room temperature is not a suitable method for preserving bacterial cultures.

Blood agar provides the necessary nutrients for the growth of fastidious bacteria.

PCR amplifies specific DNA sequences for bacterial identification.

Streaking allows for the separation and growth of individual bacterial colonies.

Direct microscopic count is not a method used for counting bacterial colonies on agar plates.

Selective media suppress the growth of unwanted organisms while allowing the growth of desired ones.

A bacterial growth curve helps understand the growth dynamics of bacteria over time.

Humidity does not directly influence bacterial growth; factors like temperature and nutrients do.

Gram staining involves the use of crystal violet and safranin to distinguish bacterial cell wall characteristics.

MIC assay determines the lowest concentration of an antibiotic that inhibits bacterial growth.

Flagella staining is not a method for assessing bacterial motility.

Electroporation is a common method for introducing foreign DNA into bacteria.

Chitin is not found in bacterial cell walls; it's a component of fungal cell walls.