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Antibiotic Susceptibility Testing Methods Quiz

#1

Which method is commonly used for antibiotic susceptibility testing?

Disk diffusion method
Explanation

It assesses antibiotic sensitivity by measuring the zone of inhibition on agar.

#2

In antibiotic susceptibility testing, what does the term 'zone of inhibition' refer to?

Area without bacterial growth around an antibiotic disk
Explanation

It indicates the effectiveness of an antibiotic in inhibiting bacterial growth.

#3

Which antibiotic susceptibility testing method is based on the measurement of bacterial turbidity?

Broth microdilution method
Explanation

It gauges bacterial growth by assessing turbidity in broth with different antibiotic concentrations.

#4

What does the term 'synergy' refer to in antibiotic susceptibility testing?

The combined effect of two antibiotics is greater than the sum of their individual effects
Explanation

It describes an enhanced antibacterial effect when antibiotics are used together.

#5

Which of the following factors can affect the accuracy of antibiotic susceptibility test results?

The presence of foreign substances in the sample
Explanation

Contaminants in the sample can interfere with accurate susceptibility testing.

#6

What does MIC stand for in the context of antibiotic susceptibility testing?

Minimum Inhibitory Concentration
Explanation

It represents the lowest concentration of an antibiotic that inhibits visible bacterial growth.

#7

Which technique measures bacterial growth as an indicator of antibiotic susceptibility?

Broth microdilution method
Explanation

It involves diluting antibiotics in broth and observing bacterial growth to determine susceptibility.

#8

Which antibiotic susceptibility testing method is based on the diffusion of antimicrobial agents through agar gel?

E-test
Explanation

It uses strips containing a gradient of antibiotic to determine MIC by observing growth inhibition.

#9

What is the main advantage of automated systems in antibiotic susceptibility testing?

They provide rapid results
Explanation

They offer faster processing and analysis of susceptibility results.

#10

What is the significance of the term 'breakpoint' in antibiotic susceptibility testing?

It indicates the concentration of antibiotic required for inhibition
Explanation

It delineates the threshold between susceptibility and resistance to a specific antibiotic.

#11

What is the purpose of the Kirby-Bauer method in antibiotic susceptibility testing?

To assess antibiotic sensitivity on agar
Explanation

It measures the diameter of zones of inhibition to determine antibiotic susceptibility.

#12

Which of the following is a rapid method for antibiotic susceptibility testing?

MALDI-TOF mass spectrometry
Explanation

It identifies bacteria and determines their susceptibility to antibiotics rapidly.

#13

Which parameter is assessed in the E-test for antibiotic susceptibility?

MIC value
Explanation

It determines the concentration of antibiotic that inhibits bacterial growth.

#14

What is the primary purpose of quality control strains in antibiotic susceptibility testing?

To ensure the accuracy and reliability of the test
Explanation

They verify that the testing process is consistent and reliable.

#15

What is the primary limitation of the agar dilution method for antibiotic susceptibility testing?

It requires specialized equipment
Explanation

It necessitates precise equipment for preparing antibiotic gradients.

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